Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 27;18:169. doi: 10.1186/s12964-020-00660-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — CDX2 was required for JARID1B-mediated Wnt/β-catenin signaling in CRC cells. a mRNA levels of CDX2 were detected by qRT-PCR. The knockdown of CDX2 expression dramatically inhibits the increase of CDX2 expression in DLD-1-shJARID1B cells. b Western blot analysis showed that the knockdown of CDX2 expression markedly rescused the increase of GSK-3β, Axin2 and p-β-catenin expression and the decrease of β-catenin, c-MYC and cyclinD1 expression induced by JARID1B knockdown. c Ubiquitinated β-catenin levels when JARID1B and CDX2 knockdown in DLD-1 cells. d Silencing CDX2 attenuated the loss of downregulating JARID1B on Wnt/β-catenin as observed by TOP-Flash luciferase assay. e The upregulation of CDX2 mRNA expression markedly inhibited the decrease in CDX2 expression observed in JARID1B-overexpression LOVO cells. f Western blot analysis showed that the levels of JARID1B overexpression and CDX2 upregulation and their effects on GSK-3β, Axin2, β-catenin, c-MYC and cyclinD1 expression in LOVO cells. g Ubiquitinated β-catenin levels when JARID1B and CDX2 over-expressed in LOVO cells. h TOP-Flash luciferase reporter assay showed the overexpression of ectopic CDX2 attenuated the increase in Wnt/β-catenin activitiy in JARID1B-overexpression LOVO cells.*p < 0.05