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Real-time PCR amplification curves for the positive control plasmid at different dilutions. Key: 1–8, plasmid diluted at 1 (1 ng/μl); 10−1 (100 pg/μl); 10−2 (10 pg/μl); 10−3 (1 pg/μl); 10−4 (100 fg/μl); 10−5 (10 fg/μl); 10−6 (1 fg/μl); and 10−7 (0.1 fg/μl), respectively
