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. 2020 Oct 27;22:256. doi: 10.1186/s13075-020-02325-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The migration and ECM secretion of chondrocytes were promoted by miR-136-5p through ELF3. a The expression of miR-136-5p in transfected chondrocytes examined by RT-qPCR. b Migration analysis of chondrocytes (× 200). c The mRNA expression of MMP-13, collagen II, aggrecan, and SOX9 in chondrocytes examined by RT-qPCR. d The protein expression of MMP-13, collagen II, aggrecan, and SOX9 in chondrocytes examined by Western blot. Asterisk symbol indicated p < 0.05 compared with the cells transfected with agomir-NC. Number sign indicated p < 0.05 compared with the cells transfected with antagomir-NC. Ampersand symbol indicated p < 0.05 compared with the cells transfected with miR-136-5p agomir. p < 0.05 indicated a significant difference. The above data were measurement data and expressed as the mean ± standard deviation. The data among groups were compared using one-way ANOVA: followed by Tukey’s post hoc test