Fig. 6.

Human KBTBD8 regulated the activity of multiple cell cycle kinases in EOC cells. a, b Blot and quantification showed that KBTBD8 knockdown decreased the level of p-MTOR, p-Akt, p-Rps6, p-Erk1/2 and p-Src in A2780 cells (CTR vs. K8-KD, p-MTOR level, 0.1240 vs. 0.05371; p-Akt level, 0.2387 vs. 0.07942; p-Rps6 level, 0.3059 vs. 0.006376; p-Erk1/2 level, 0.1456 vs. 0.08771; p-Src level, 0.1378 vs. 0.02054), while KBTBD8 knockdown did not decreased the level of total MTOR, Akt, Rps6, Erk1/2, and Src level in A2780 cells. c Co-IP and blot showed that cul3 and KBTBD8 interacted with each other. d, e Blot and quantification showed that KBTBD8 knockdown significantly diminished the overall level of ubiquitination (ubiquitination level, CTR vs. K8-KD, 3.085 vs. 2.391). A.U., arbitrary unit. *Indicates p < 0.05, ***indicates p < 0.001, ****indicates p < 0.0001