Figure S2.

Spatio-temporal Organization of Subcellular Organelles and Coronavirus during Infection, Related to Figure 1

(A) The density of MHV (M_J1.3) gold particles per 0.5μm² area (n=10 areas with and n=10 areas without virions) of infected cells is plotted.

(B) HeLa-mCC1a cells, infected with MHV, fixed (6 h and 12 h pi), and coimmunostained with anti-E(green) and anti-S (red) antibodies.

(C) HeLa-mCC1a cells, infected with MHV, fixed (6 h and 12 h pi), and coimmunostained with anti-E(green) and anti-MHV(M_J1.3) (red) antibodies.

(D) HeLa-mCC1a cells infected with MHV, fixed (6 h, 9 h and 12 h pi)) and coimmunostained with anti-TGN46 (green) and anti-MHV(M_J1.3) (red).

(E) HeLa-mCC1a cells infected with MHV, fixed (5 h and 14 h pi) and coimmunostained with anti-Golgin97 (green) and anti-MHV(M_J1.3) (red). Scale bar 10 μm.

(F) HeLa-mCC1a cells infected with MHV, fixed (6 h and 10 h pi) and coimmunostained with anti-mannosidase II (green) and anti-MHV(M_J1.3) (red).

(G) MHV-infected cells, washed, fixed (6 h and 12 h pi), and coimmunostained with anti-cathepsin D (green) and anti-MHV(M_J1.3) (red) antibodies. Arrows point to Cathepsin⁺/MHV⁺ puncta.

(H) HeLa-mCC1a cells infected with MHV, fixed at 12 h pi and coimmunostained with anti-LAMP1 (blue), anti-MHV(M_J1.3) (red) and anti-Atlastin 3 (green) antibodies. White arrows point to LAMP1⁺/MHV⁺ puncta; turquoise arrows point to Atlastin-3⁺/MHV⁺ puncta.

Representative images are shown. Scale bar 5μm unless indicated.