Table 3. Estimated Secondary Structure Content (%) of PGA Determined from Fitting to the CD Spectraa.
secondary structure |
pH |
|||||||
---|---|---|---|---|---|---|---|---|
3.6 | 4.3 | 5.1 | 5.4 | 5.9 | 7.2 | 10.4 | ||
helix | helix1 (regular) | 30.9 | 58.4 | 56.8 | 48.5 | 20.1 | 0.0 | 0.0 |
helix2 (distorted) | 10.6 | 19.2 | 16.7 | 15.1 | 9.2 | 0.0 | 0.0 | |
antiparallel | anti1 (left-twisted) | 2.7 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
anti2 (relaxed) | 8.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
anti3 (right-twisted) | 0.0 | 0.0 | 0.0 | 0.4 | 15.3 | 42.3 | 43.1 | |
parallel | 15.7 | 1.5 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | |
turn | 16.5 | 9.5 | 7.4 | 10.1 | 13.1 | 14.0 | 13.3 | |
others | 15.7 | 11.4 | 19.1 | 26.0 | 42.4 | 43.8 | 43.6 | |
RMSD | 0.1114 | 0.1902 | 0.1442 | 0.0636 | 0.1435 | 0.728 | 0.89 | |
NRMSD | 0.00561 | 0.00589 | 0.0048 | 0.0026 | 0.01422 | 0.06347 | 0.07192 |
The fitting has been completed by dividing the spectrum into eight secondary structure elements following ref (83).