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. 2020 Oct 15;16(10):e1008689. doi: 10.1371/journal.pgen.1008689

Fig 3. Target-adjacent mutagenesis is dependent on Poζ.

Fig 3

A) Effect of SSTR on a URA3 gene integrated near the site of HO cleavage. Mutations in URA3 following SSTR were collected by replica plating survivors on galactose media onto 5-FOA medium. B) The increase in mutation rate in SSTR over the spontaneous mutation rate (determined by a fluctuation analysis) was determined at the indicated locations surrounding the DSB site. C) Effect of deleting Rev1 and Rev3 components of Polζ. Significance determined using an unpaired t-test with Welch’s correction, comparing mutants to WT. Error bars refer to standard error of the mean. Spontaneous mutation rate determine by fluctuation analysis, n = 10. SSTR mutation rate, n = 3.