Fig 5. COX-2 regulates vGPCR angiogenicity.

A) Cells expressing vGPCR and corresponding controls were treated or not with NS398 (10 mM) and inoculated I.D. into both flanks of nude mice (n = 5). Mice were sacrificed after 5 days and the area of inoculation was photographed under a dissection microscope. B) Neovessel formation determined by morphometric analysis. The bar graphs show the mean microvessel density (vessels/mm²) for NS398 pre-treated (grey bars) or untreated (black bars) cells +/-SD. Total n (both flanks) = 10. (*) Indicates significant differences between groups injected with vGPCR-transformed cells and NIH3T3 control cells (P<0.05). (#) Indicates significant differences between groups injected with NS398-treated and non-treated vGPCR-transformed cells (P<0.01).