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. 2020 Oct 23;12:10505–10519. doi: 10.2147/CMAR.S272324

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© 2020 Liu et al.

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Circ_0000260 regulated CDDP chemoresistance of HGC27/CDDP and AGS/CDDP cells by targeting miR-129-5p. (A) The efficiency of miR-129-5p inhibitor was examined by qRT-PCR. HGC27/CDDP and AGS/CDDP cells were next transfected with si-circ#2, si-NC, si-circ#2+anti-miR-129-5p or si-circ#2+anti-NC. In these transfected cells, (B) Cell proliferation of HGC27/CDDP and HGC27 cells after the treatment of CDDP was assessed by CCK-8 assay. (C) cell proliferation was monitored using CCK-8 assay. (D) Colony formation ability was assessed by colony formation assay. (E) Cell apoptosis was monitored by flow cytometry assay. (F and G) Cell migration and cell invasion were assessed by scratch assay and transwell assay, respectively. (H) Cell adhesion capacity was detected by cell adhesion assay. (I) The expression of fibronectin and vitronectin was quantified by Western blot. *P<0.05, **P<0.01, ***P<0.001.