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. 2020 Oct 23;12:10505–10519. doi: 10.2147/CMAR.S272324

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© 2020 Liu et al.

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MMP11 was a target of miR-129-5p and was upregulated in GAC tissues. (A) Venn diagram was depicted to screen mRNAs detected among starBase, miRWalk and GEPIA database, and their expression in HGC27/CDDP and AGS/CDDP cells transfected with miR-129-5p or miR-NC was detected by qRT-PCR. (B) The expression of MMP11 in GAC and normal tissues was obtained from GEPIA database, and MMP11 expression was also measured in clinical tissues by qRT-PCR. (C) The correlation between MMP11 expression and miR-129-5p expression in GAC tissues was analyzed by Spearman’s test. (D) The expression of MMP11 expression in tumor tissues and normal tissues was detected by Western blot. (E and F) The expression of MMP11 in CDDP-sensitive and CDDP-resistant GAC tissues was detected by qRT-PCR and Western blot. (G and H) The expression of MMP11 in CDDP-sensitive and CDDP-resistant GAC cells was detected by qRT-PCR and Western blot. (I) MUT-MMP11 3ʹUTR sequence was designed according to WT-MMP11 3ʹUTR sequence. (J) The relationship between miR-129-5p and MMP11 was verified by dual-luciferase reporter assay. (K and L) The expression of MMP11 in HGC27/CDDP and AGS/CDDP cells with miR-129-5p overexpression or knockdown was detected by qRT-PCR and Western blot. *P<0.05, **P<0.01, ***P<0.001.