Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. rerio) | scospondinicm13 | Cantaut-Belarif et al., 2018 | ZFIN : ZDB-ALT-181113–3 | |
| Genetic reagent (D. rerio) | scospondinicm15 | Cantaut-Belarif et al., 2018 | ZFIN: ZDB-ALT- 181113–4 | |
| Genetic reagent (D. rerio) | pkd2l1icm02 | Böhm et al., 2016 | ZFIN : ZDB-ALT-160119–6 | |
| Genetic reagent (D. rerio) | Tg(pkd2l1:GCaMP5G)icm07Tg | Böhm et al., 2016 | ZFIN: ZDB-ALT-160119–4 | |
| Genetic reagent (D. rerio) | Tg(pkd2l1:GAL4)icm10Tg | Fidelin et al., 2015 | ZFIN: ZBD-ALT-150324–1 | |
| Genetic reagent (D. rerio) | Tg(UAS:mCherry) | Robles et al., 2014 | ZFIN: ZBD-ALT-130702–1 | |
| Genetic reagent (D. rerio) | scospondinut24Tg | Troutwine et al., 2020 | R. S. Gray lab | |
| Recombinant DNA reagent | pCS2-urp2 plasmid | G. Pézeron, this work | Used for RNA synthesis | |
| Recombinant DNA reagent | pCS2-Ras-eGFP plasmid | Ségalen et al., 2010 | Used for RNA synthesis | |
| Sequence-based reagent | URP2_BamHI_F gcgcgcGGATCC gtatctgtagaatctgctttgctgc |
This work | forward oligonucleotide used for urp2 cloning |
|
| Sequence-based reagent | URP2_XbaI_R gcgcgcTCTAGA ggcagagggtcagtcgtgttat |
This work | reverse oligonucleotide used for urp2 cloning |
|
| Sequence-based reagent |
urp2 forward: CCACCGGATCACCATCATTACC |
This work | qPCR oligonucleotide | |
| Sequence-based reagent |
urp2 reverse: GATGCCACCGCTGTCTATAGTG |
This work | qPCR oligonucleotide | |
| Sequence-based reagent |
urp1 forward: TGCGCTGCCTCTGTATTCAG |
This work | qPCR oligonucleotide | |
| Sequence-based reagent |
urp1 reverse: CTTTGTCCGTCTTCAACCTCTG |
This work | qPCR oligonucleotide | |
| Sequence-based reagent |
pkd2l1 forward: GCGAACTATGCCCAATGAGG |
This work | qPCR oligonucleotide | |
| Sequence-based reagent |
pkd2l1 reverse: TCTCAAAGCTGTTCCCCACA |
This work | qPCR oligonucleotide | |
| Sequence-based reagent |
lsm12b forward: GAGACTCCTCCTCCTCTAGCAT |
This work | qPCR oligonucleotide | |
| Sequence-based reagent |
lsm12b reverse: GATTGCATAGGCTTGGGACAAC |
This work | qPCR oligonucleotide | |
| Antibody | Anti-Reissner fiber, rabbit, polyclonal |
Didier et al., 1995 | Courtesy of S. Gobron |
Dilution 1:200 |
| Antibody | Anti-GFP, chicken, polyclonal | Abcam, Cat# ab13970 | RRID:AB_300798 | Dilution 1:500 |
| Antibody | Anti-Pkd2l1, rabbit, polyclonal | Sternberg et al., 2018 | Dilution 1:200 | |
| Antibody | Anti-norepinephrine, rabbit, polyclonal |
Millipore, Cat# AB120 | RRID:AB_90481 | Dilution 1:100 |
| Antibody | Anti-Adrb2, rabbit, polyclonal | ThermoFischer Scientific, Cat# PA5-80323 |
RRID:AB_2787652 | Dilution 1:200 |
| Antibody | Alexa Fluor-488 goat anti chicken IgG (H+L) |
Molecular Probes, Cat# A-11039 |
RRID:AB_142924 | Dilution 1:500 |
| Antibody | Alexa Fluor-568 goat anti-rabbit IgG (H+L) |
Molecular Probes, Cat# A-11036 |
RRID:AB_10563566 | Dilution 1:500 |
| Antibody | Alexa Fluor-488 donkey anti rabbit IgG (H+L) |
Molecular Probes, Cat# A-21206 |
RRID:AB_2535792 | Dilution 1:500 |
| Chemical compound, drug | DL-Norepinephrine hydrochloride |
Sigma | Cat# A7256 | Dilution to 3 mM |
| Chemical compound, drug | +- Epinephrine hydrochloride |
Sigma | Cat# E4642 | Dilution to 3 mM |
| Chemical compound, drug | MS 222 | Sigma | Cat# E10521 | Dilution to 0.2% w/v |
| Chemical compound, drug | alpha-bungarotoxin | Tocris | Cat# 2133 | Dilution to 500 µM |
| Software, algorithm | MATLAB | MathWorks | RRID:SCR_001622 | |
| Software, algorithm | Prism | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | Fiji | Schindelin et al., 2012 | RRID:SCR_002285 | |
| Software, algorithm | Imaris | Oxford Instruments | RRID:SCR_007370 |