Table 1. Homology of the C. crescentus enzymes involved in the pseudaminic acid biosynthesis pathway to S. fredii NGR234 and C. jejuni proteins.
| C. crescentus | S. fredii NGR234 | C. jejuni NCTC 11168 | Enzymatic activity |
|---|---|---|---|
| FlmA (CCNA_00233) |
RkpL 52% id; 68% sim |
PseB 57% id; 72% sim |
Dehydratase |
| FlmB (CCNA_00234) |
RkpM 44% id; 58% sim |
PseC 34% id; 52% sim |
Aminotransferase |
| FlmH (CCNA_01523) |
Rkp3_013†
26% id; 37% sim |
PseH‡
26% id; 50% sim |
N-acetyltransferase |
| FlmD (CCNA02947) |
RkpO 36% id; 49% sim |
PseG 22% id; 41% sim |
UDP-sugar hydrolase |
| NeuB (CCNA_02961) |
RkpQ 56% id; 70% sim |
PseI 43% id; 59% sim |
Pseudaminic acid synthase |
| FlmC§
(CCNA_02946) |
RkpN | PseF | Cytidyltransferase |
| FlmG (CCNA_01524) |
- | - | Protein Glycosyltransferase |
* Although C. crescentus genomes encodes for several putative acetyltransferases, only CCNA_01531 has significant homology to FlmH (35% identity [id] and 54% similarity [sim]).
† FlmH aligns to Rkp3_013 only for about 50% of its length.
‡ FlmH aligns to PseH for about 75% of its length.
§ FlmC shows no homology to RkpN or PseF; RkpN and PseF share 46% identity and 68% similarity.