Appendix 1—key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | c587-GAL4 | Manseau et al., 1997 | FBal0150629 RRID:BDSC_67747 |
A gift from Hiroko Sano, Kurume University, Japan |
| Genetic reagent (D. melanogaster) | OambΔ | This paper | Detail described in Figure 1—figure supplement 1F | |
| Genetic reagent (D. melanogaster) | nAChRa1228 | This paper | Detail described in Figure 6—figure supplement 2A | |
| Genetic reagent (D. melanogaster) | nAChRa1326 | This paper | Detail described in Figure 6—figure supplement 2A | |
| Genetic reagent (D. melanogaster) | EcRA483T | Bloomington Drosophila Stock Center | BDSC: #5799 RRID:BDSC_5799 FBal0083501 |
|
| Genetic reagent (D. melanogaster) | EcRM554fs | Bloomington Drosophila Stock Center | BDSC: #4894 RRID:BDSC_4894 FBal0083490 |
|
| Genetic reagent (D. melanogaster) | Vkg::GFP | KYOTO stock center | DGRC #110692 RRID:DGGR_110692 FBal0286156 |
|
| Genetic reagent (D. melanogaster) | Dad-LacZ | Tsuneizumi et al., 1997 | FBal0065787 RRID:DGGR_118114 |
A gift from Yoshiki Hayashi, University of Tsukuba, Japan |
| Genetic reagent (D. melanogaster) | R44E10-GAL4 | Deady and Sun, 2015 | FBal0252601 PMID:26473732 |
A gift from Jianjun Sun, University of Connecticut, USA |
| Genetic reagent (D. melanogaster) | RS-GAL4 | Lee et al., 2009 | FBal0263794 PMID:19262750 |
A gift from Kyung-An Han, Pennsylvania State University, USA |
| Genetic reagent (D. melanogaster) | nSyb-GAL4 | Bloomington Drosophila Stock Center | BDSC: #51941 RRID:BDSC_51941 FBti0154973 |
FACS (5 ul per test) |
| Genetic reagent (D. melanogaster) | nSyb-GAL80 | Harris et al., 2015 | PMID:26193122 | A gift from James W. Truman, Janelia Research Campus, USA |
| Genetic reagent (D. melanogaster) | tj-GAL4 | KYOTO stock center | DGRC: #104055 RRID:DGGR_104055 FBti0034540 |
|
| Genetic reagent (D. melanogaster) | R13C06-GAL4 | Bloomington Drosophila Stock Center | BDSC: #47860 RRID:BDSC_47860 FBal0249828 |
|
| Genetic reagent (D. melanogaster) | 109–30 GAL4 | Bloomington Drosophila Stock Center | BDSC: #7023 RRID:BDSC_7023 FBti0027548 |
|
| Genetic reagent (D. melanogaster) | c355-GAL4 | Bloomington Drosophila Stock Center | BDSC: #3750 RRID:BDSC_3750 FBti0002591 |
|
| Genetic reagent (D. melanogaster) | c306-GAL4 | Bloomington Drosophila Stock Center | BDSC: #3743 RRID:BDSC_3743 FBal0048787 |
|
| Genetic reagent (D. melanogaster) | slbo-GAL4 | Bloomington Drosophila Stock Center | BDSC: #6458 RRID:BDSC_6458 FBst0006458 |
|
| Genetic reagent (D. melanogaster) | bab1-GAL4 | Bolívar et al., 2006 | FBal0242654 PMID:17013875 |
A gift from Satoru Kobayashi, University of Tsukuba, Japan |
| Genetic reagent (D. melanogaster) | nos-GAL4 | KYOTO stock center | DGRC: #107748 RRID:DGGR_107748 FBst0306396 |
|
| Genetic reagent (D. melanogaster) | tub > FRT > GAL80>FRT | Bloomington Drosophila Stock Center | BDSC: #38879 RRID:BDSC_38879 FBti0147580 |
|
| Genetic reagent (D. melanogaster) | OambKI-RD-GAL4 |
Deng et al., 2019
Bloomington Drosophila Stock Center |
BDSC: #84677 RRID:BDSC_84677 FBti0209942 |
|
| Genetic reagent (D. melanogaster) | Oamb-KI-T2A-GAL4 | Kondo et al., 2020 | PMID:31914394 | |
| Genetic reagent (D. melanogaster) | nAChRα1-T2A-GAL4 | Kondo et al., 2020 | PMID:31914394 | |
| Genetic reagent (D. melanogaster) | nAChRα2-T2A-GAL4 | Kondo et al., 2020 | PMID:31914394 | |
| Genetic reagent (D. melanogaster) | nAChRα3-T2A-GAL4 | Kondo et al., 2020 | PMID:31914394 | |
| Genetic reagent (D. melanogaster) | nAChRβ1-T2A-GAL4 | Kondo et al., 2020 | PMID:31914394 | |
| Genetic reagent (D. melanogaster) | nAChRβ2-T2A-GAL4 | Kondo et al., 2020 | PMID:31914394 | |
| Genetic reagent (D. melanogaster) | ChaT-GAL4 | Bloomington Drosophila Stock Center | BDSC: #6793 RRID:BDSC_6793 FBst0006793 |
|
| Genetic reagent (D. melanogaster) | ppk-GAL4 | Grueber et al., 2007 | FBtp0039691 PMID:17164414 |
A gift from Hiroko Sano, Kurume University, Japan |
| Genetic reagent (D. melanogaster) | SPSNs-LexA | Feng et al., 2014 | FBtp0110869 PMID:24991958 |
A gift from Young-Joon Kim, Gwangju Institute of Science and Technology, South Korea |
| Genetic reagent (D. melanogaster) | 20xUAS-6xGFP | Bloomington Drosophila Stock Center | BDSC: #52261 RRID:BDSC_52261 FBst0052261 |
|
| Genetic reagent (D. melanogaster) | UAS-GFP;UAS-mCD8::GFP | Ito et al., 1997; Lee and Luo, 1999 | FBtp0002652 PMID:9043058 PMID:10457015 |
A gift from Kei Ito, University of Cologne, Germany |
| Genetic reagent (D. melanogaster) | UAS-Stinger | Bloomington Drosophila Stock Center | BDSC: #84277 RRID:BDSC_84277 |
|
| Genetic reagent (D. melanogaster) | UAS-mCD8::RFP | Bloomington Drosophila Stock Center | BDSC: #32219 RRID:BDSC_32219 FBti0131967 |
|
| Genetic reagent (D. melanogaster) | UAS-CsChrimson | Bloomington Drosophila Stock Center | BDSC: #55134 RRID:BDSC_55134 FBti0160571 |
|
| Genetic reagent (D. melanogaster) | UAS-Insp3R | Bloomington Drosophila Stock Center | BDSC: #30742 RRID:BDSC_30742
FBti0129829 |
|
| Genetic reagent (D. melanogaster) | UAS-OambK3 | Lee et al., 2009 | FBtp0069415 PMID:19262750 |
A gift from Kyung-An Han, Pennsylvania State University, USA |
| Genetic reagent (D. melanogaster) | UAS-Timp | Bloomington Drosophila Stock Center | BDSC: #58708 RRID:BDSC_58708 FBti0164930 |
A gift from Andrea Page-McCaw, Vanderbilt University, USA |
| Genetic reagent (D. melanogaster) | UAS-nAChRα1 | This paper | Detail described in Material and method | |
| Genetic reagent (D. melanogaster) | UAS > stop > dTrpA1mcherry | von Philipsborn et al., 2011 | FBtp0064577 PMID:21315261 |
A gift from Daisuke Yamamoto, Advanced ICT Research Institute, National Institute of Information and Communications Technology, Japan |
| Genetic reagent (D. melanogaster) | UAS > stop > TNT | von Philipsborn et al., 2011 | FBtp0020863 PMID:21315261 |
A gift from Daisuke Yamamoto, Advanced ICT Research Institute, National Institute of Information and Communications Technology, Japan |
| Genetic reagent (D. melanogaster) | UAS > stop > TNTin | von Philipsborn et al., 2011 | FBtp0020863 PMID:21315261 |
A gift from Daisuke Yamamoto, Advanced ICT Research Institute, National Institute of Information and Communications Technology, Japan |
| Genetic reagent (D. melanogaster) | dsx-FLP | Rezával et al., 2014 | FBal0296301 PMID:24631243 |
A gift from Daisuke Yamamoto, Advanced ICT Research Institute, National Institute of Information and Communications Technology, Japan |
| Genetic reagent (D. melanogaster) | TRiC; UAS-mCD8::RFP, LexAop2-mCD8::GFP;nSyb-MKII::nlsLexADBDo;UAS-p65AD::CaM | Bloomington Drosophila Stock Center | BDSC: #61679 RRID:BDSC_61679 FBst0061679 |
|
| Genetic reagent (D. melanogaster) | ppk-eGFP | Grueber et al., 2003 | FBtp0041053 PMID:12699617 |
A gift from Tadashi Uemura, Kyoto University, Japan |
| Genetic reagent (D. melanogaster) | LexAop-Kir2.1 | Feng et al., 2014 | FBtp0110870 PMID:24991958 |
A gift from Young-Joon Kim, Gwangju Institute of Science and Technology, South Korea |
| Genetic reagent (D. melanogaster) | UAS-LacZRNAi | Kennerdell and Carthew, 2000 | FBtp0016505 PMID:10932163 |
A gift from Masayuki Miura, The University of Tokyo, Japan |
| Genetic reagent (D. melanogaster) | UAS-OambRNAi1 | Bloomington Drosophila Stock Center | BDSC: #31171 RRID:BDSC_31171 FBst0031171 |
|
| Genetic reagent (D. melanogaster) | UAS-OambRNAi2 | Bloomington Drosophila Stock Center | BDSC: #31233 RRID:BDSC_31233 FBst0031233 |
|
| Genetic reagent (D. melanogaster) | UAS-OambRNAi3 | Vienna Drosophila Resource Center | VDRC: #106511 FBst0478335 |
|
| Genetic reagent (D. melanogaster) | UAS-Octβ1RRNAi | Vienna Drosophila Resource Center | VDRC: #110537 FBst0482104 |
|
| Genetic reagent (D. melanogaster) | UAS-Octβ2RRNAi | Vienna Drosophila Resource Center | VDRC: #104524 FBst0476382 |
|
| Genetic reagent (D. melanogaster) | UAS-Octβ3RRNAi | Vienna Drosophila Resource Center | VDRC: #101189 FBst0473062 |
|
| Genetic reagent (D. melanogaster) | UAS-Insp3RRNAi | Bloomington Drosophila Stock Center | BDSC: #25937 FBst0025937 |
|
| Genetic reagent (D. melanogaster) | UAS-EcRRNAi | Vienna Drosophila Resource Center | VDRC: #37059 FBst0461818 |
|
| Genetic reagent (D. melanogaster) | UAS-Mmp2RNAi1 | Bloomington Drosophila Stock Center | BDSC: #31371 RRID:BDSC_31371 FBst0031371 |
|
| Genetic reagent (D. melanogaster) | UAS-Mmp2RNAi2 | Vienna Drosophila Resource Center | VDRC: #330203 FBst0490996 |
|
| Genetic reagent (D. melanogaster) | UAS-TimpRNAi1 | Bloomington Drosophila Stock Center | BDSC: #61294 RRID:BDSC_61294 FBst0061294 |
|
| Genetic reagent (D. melanogaster) | UAS-TimpRNAi2 | Vienna Drosophila Resource Center | VDRC: #109427 FBst0481116 |
|
| Genetic reagent (D. melanogaster) | UAS-Tdc2RNAi1 | Vienna Drosophila Resource Center | VDRC: #330541 FBst0492256 |
|
| Genetic reagent (D. melanogaster) | UAS-Tdc2RNAi2 | Bloomington Drosophila Stock Center | BDSC: #25871 RRID:BDSC_25871 FBst0025871 |
|
| Genetic reagent (D. melanogaster) | UAS-TβhRNAi1 | Vienna Drosophila Resource Center | VDRC: #107070 FBst0478893 |
|
| Genetic reagent (D. melanogaster) | UAS-TβhRNAi2 | Bloomington Drosophila Stock Center | BDSC: #67968 RRID:BDSC_67968 FBst0067968 |
|
| Genetic reagent (D. melanogaster) | UAS-ChATRNAi1 | Vienna Drosophila Resource Center | VDRC: #330291 FBst0490951 |
|
| Genetic reagent (D. melanogaster) | UAS-ChATRNAi2 | Bloomington Drosophila Stock Center | BDSC: #25856 RRID:BDSC_25856 FBst0025856 |
|
| Genetic reagent (D. melanogaster) | UAS-nAChRα1RNAi | Vienna Drosophila Resource Center | VDRC #48159 FBst0467755 |
|
| Genetic reagent (D. melanogaster) | UAS-nAChRα2RNAi | Vienna Drosophila Resource Center | VDRC: #101760 FBst0473633 |
|
| Genetic reagent (D. melanogaster) | UAS-nAChRα3RNAi | Vienna Drosophila Resource Center | VDRC: #101806 | |
| Genetic reagent (D. melanogaster) | UAS-nAChRβ1RNAi | Vienna Drosophila Resource Center | VDRC: #106570 FBst0478394 |
|
| Genetic reagent (D. melanogaster) | UAS-nAChRβ2RNAi | Vienna Drosophila Resource Center | VDRC: #109450 FBst0481138 |
|
| Genetic reagent (D. melanogaster) | UAS-nvdRNAi1 | Yoshiyama et al., 2006 | FBal0193613 PMID:16763204 |
|
| Genetic reagent (D. melanogaster) | UAS-nvdRNAi2 | Yoshiyama et al., 2006 | FBal0193614 PMID:16763204 |
|
| Chemical, compound, drug | Octopamine | Sigma-Aldrich | #O0250 | |
| Chemical, compound, drug | Schneider’s Drosophila medium | Thermo Fisher Scientific | #21720024 | |
| Chemical, compound, drug | 20-hydroxyecdysone | Enzo Life Sciences | ALX-370–012 | |
| Antibody | anti-GFP (chicken polyclonal) | Abcam | #ab13970 | 1:4000 dilution |
| Antibody | anti-RFP (rabbit polyclonal) | Medical and Biological Laboratories | #PM005 | 1:2000 dilution |
| Antibody | anti-Hts 1B1 (mouse monoclonal) | Developmental Studies Hybridoma Bank | 1:50 dilution | |
| Antibody | anti-DE-cadherin DCAD2 (rat monoclonal) | Developmental Studies Hybridoma Bank | 1:50 dilution | |
| Antibody | anti-pH3 (rabbit polyclonal) | Merck Millipore | #06–570 | 1:2000 dilution |
| Antibody | anti-pMad (rabbit polyclonal) | Abcam | #ab52903 | 1:2000 dilution |
| Antibody | anti-Lamin C LC28.26 (mouse monoclonal) | Developmental Studies Hybridoma Bank | 1:10 dilution | |
| Antibody | anti-cleaved Dcp-1 (rabbit polyclonal) | Cell Signaling Technology | #9578 | 1:1000 dilution |
| Antibody | anti-Vasa (rat monoclonal) | Developmental Studies Hybridoma Bank | 1:50 dilution | |
| Antibody | anti-LacZ 40-1a (mouse monoclonal) | Developmental Studies Hybridoma Bank | 1:50 dilution | |
| Antibody | anti-Tdc2 (rabbit polyclonal) | Abcam | #ab128225 | 1:2000 dilution |
| Antibody | Alexa Fluor 546 phalloidin | Thermo Fisher Scientific | #A22283 | 1:200 dilution |
| Antibody | Alexa Fluor 633 phalloidin | Thermo Fisher Scientific | #A22284 | 1:200 dilution |
| Chemical, compound, drug | FluorSave reagent | Merck Millipore | #345789 | |
| Chemical, compound, drug | all trans-Retinal | Sigma-Aldrich | #R2500 | |
| Software, algorithm | ImageJ |
https://imagej.nih.gov/ij; RRID:SCR_003070
PMID:22930834 |
||
| Software, algorithm | R | RRID:SCR_001905 |