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. 2020 Sep 29;9:e57761. doi: 10.7554/eLife.57761

Figure 4. YAP1/WWTR1 knockout impairs proliferation and induces apoptosis in dependent cells.

Figure 4.

(A) Knocking out YAP1 or WWTR1 significantly inhibited cell proliferation in the respective dependent cell lines. Cell viability of HSC-2 (a YAP1- and WWTR1-non-dependent cell line) was not affected by depletion of either YAP1 or WWTR1. Experiments are repeated twice in technical triplicates. (n = 3 technical repeats for two biological repeats). Data shown as mean ± SD. *p<0.01; **p<0.001. (B) Apoptosis assay revealed that YAP1 depletion in YAP1-dependent ORL-48 resulted in significant increase in apoptotic cells while WWTR1 depletion in WWTR1-dependent ORL-214 resulted in an increase in apoptotic cells. No significant changes in apoptotic cells were observed in HSC-2 when either YAP1 or WWTR1 was depleted. Experiments are repeated twice in technical triplicates. Data of one experiment are shown as mean ± SD (n = 3 technical repeats). *p<0.01; **p<0.001.

Figure 4—source data 1. Data for cell viability and apoptosis assays.