Appendix 1—key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Wnk1 | Ensembl | ENSMUSG00000045962 | |
| Gene (Mus musculus) | Oxsr1 | Ensembl | ENSMUSG00000036737 | |
| Gene (Mus musculus) | Stk39 | Ensembl | ENSMUSG00000027030 | |
| Gene (Mus musculus) | Slc12a2 | Ensembl | ENSMUSG00000024597 | |
| Gene (Mus musculus) | Myc | Ensembl | ENSMUSG00000022346 | |
| Genetic reagent (Mus musculus) | Wnk1tm1Clhu; Wnk1fl |
Xie et al., 2009
doi:10.2353/ajpath.2009.090094 |
RRID:MGI:4360972 | |
| Genetic reagent (Mus musculus) | Wnk1tm1.1Clhu; Wnk1- |
Köchl et al., 2016
doi:10.1038/ni.3495 |
RRID:MGI:6286269 | |
| Genetic reagent (Mus musculus) | Oxsr1tm1.1Ssy; Oxsr1fl |
Lin et al., 2011
doi:10.1073/pnas.1107452108 |
RRID:MGI:5297491 | |
| Genetic reagent (Mus musculus) | Tg(CD2-icre)4Kio; CD2Cre |
de Boer et al., 2003
doi:10.1002/immu.200310005 |
RRID:MGI:2449947 | |
| Genetic reagent (Mus musculus) | Wnk1tm1.1Tyb; Wnk1D368A |
Köchl et al., 2016
doi:10.1038/ni.3495 |
RRID:MGI:6286266 | |
| Genetic reagent (Mus musculus) | Gt(ROSA)26Sortm1(cre/ERT2)Thl; ROSA26CreERT2, RCE |
de Luca et al., 2005
doi:10.1172/JCI24059 |
RRID:MGI:3701992 | |
| Genetic reagent (Mus musculus) | Oxsr1tm1.1Arte; Oxsr1T185A |
Rafiqi et al., 2010
doi:10.1002/emmm.200900058 |
RRID:MGI:5462099 | |
| Genetic reagent (Mus musculus) | Stk39tm1.1Arte; Stk39T243A |
Rafiqi et al., 2010
doi:10.1002/emmm.200900058 |
RRID:MGI:5462098 | |
| Genetic reagent (Mus musculus) | Slc12a2tm1Ges; Slc12a2- |
Flagella et al., 1999
doi:10.1074/jbc.274.38.26946 |
RRID:MGI:1935144 | |
| Genetic reagent (Mus musculus) | Rag2tm1Fwa; Rag2- |
Shinkai et al., 1992
doi:10.1016/0092-8674(92)90029 C |
RRID:MGI:1858556 | |
| Genetic reagent (Mus musculus) | Rag1tm1Mom; Rag1- |
Mombaerts et al., 1992
doi:10.1016/0092-8674(92)90030 G |
RRID:MGI:1857241 | |
| Genetic reagent (Mus musculus) | C57BL/6J.129S4-Tg(Prm-cre)70Og |
O'Gorman et al., 1997
doi:10.1073/pnas.94.26.14602 |
RRID:MGI:2388049 | |
| Cell line (Mus musculus) | OP9-DL1 |
Schmitt and Zúñiga-Pflücker, 2002
doi:10.1016/s1074-7613 (02)00474–0 |
RRID:CVCL_B218 | |
| Cell line (Homo-sapiens) | Platinum E (Plat-E) |
Morita et al., 2000
doi:10.1038/sj.gt.3301206 |
RRID:CVCL_B488 | Retroviral packaging cell line |
| Recombinant DNA reagent | pMIGR1 (plasmid) |
Pear et al., 1998
doi:10.1182/blood.V92.10.3780 |
RRID:Addgene_27490 | Retroviral construct encoding GFP |
| Recombinant DNA reagent | pMIGR1-MYC (plasmid) |
Luo et al., 2005
doi:10.1182/blood-2005-02-0734 |
Retroviral construct encoding MYC and GFP | |
| Recombinant DNA reagent | pMIGR1-MYCT58A (plasmid) | This paper | Retroviral construct encoding MYC-T58A and GFP | |
| Antibody | anti-B220-biotin (RA3-6B2) (Rat monoclonal) | Invitrogen | Cat# 13-0452-86; RRID:AB_466451 | FACS (1:200) |
| Antibody | anti-B220-FITC (RA3-6B2) (Rat monoclonal) | Biolegend | Cat# 103206; RRID:AB_312991 | FACS (1:200) |
| Antibody | anti-active Caspase 3-PE (C92-605) (Rabbit monoclonal) | BD Pharmingen | Cat# 550821; RRID:AB_393906 | FACS (1:50) |
| Antibody | anti-CD3ε-biotin (2C11) (Armenian hamster monoclonal) | Biolegend | Cat# 100304; RRID:AB_312669 | FACS (1:200) |
| Antibody | anti-CD3ε-FITC (2C11) (Armenian hamster monoclonal) | eBioscience | Cat# 11-0031-85; RRID:AB_464883 | FACS (1:200) |
| Antibody | anti-CD3ε purified, endotoxin low (2C11) (Armenian hamster monoclonal) | Tonbo | Cat# 40–0031 M001; RRID:AB_464883 | IV injection |
| Antibody | anti-CD4-biotin (RM4-4) (Rat monoclonal) | eBioscience | Cat# 13-0043-85; RRID:AB_466334 | FACS (1:200) |
| Antibody | anti-CD4-FITC (GK1.5) (Rat monoclonal) | eBioscience | Cat# 11-0041-86; RRID:AB_464894 | FACS (1:200) |
| Antibody | anti-CD4-BV421 (RM4-5) (Rat monoclonal) | Biolegend | Cat# 100544; RRID:AB_11219790 | FACS (1:200) |
| Antibody | anti-CD4-PerCP (RM4-5) (Rat monoclonal) | Biolegend | Cat# 100538; RRID:AB_893325 | FACS (1:200) |
| Antibody | anti-CD4-APC-CY7 (GK1.5) (Rat monoclonal) | BD Pharmingen | Cat# 552051; RRID:AB_394331 | FACS (1:200) |
| Antibody | anti-CD8-biotin (53–6.7) (Rat monoclonal) | Biolegend | Cat# 100704; RRID:AB_312743 | FACS (1:200) |
| Antibody | anti-CD8-FITC (53–6.7) (Rat monoclonal) | Biolegend | Cat# 100706; RRID:AB_312745 | FACS (1:200) |
| Antibody | anti-CD8-BV421 (53–6.7) (Rat monoclonal) | Biolegend | Cat# 100738; RRID:AB_11204079 | FACS (1:200) |
| Antibody | anti-CD8-APC (53–6.7) (Rat monoclonal) | eBioscience | Cat# 17-0081-83; RRID:AB_469336 | FACS (1:200) |
| Antibody | anti-CD8-PE-CY7 (53–6.7) (Rat monoclonal) | Biolegend | Cat# 100722; RRID:AB_312761 | FACS (1:200) |
| Antibody | anti-CD11b-biotin (M1/70) (Rat monoclonal) | Biolegend | Cat# 101204; RRID:AB_312787 | FACS (1:200) |
| Antibody | anti-CD11b-FITC (M1/70) (Rat monoclonal) | Biolegend | Cat# 101206; RRID:AB_312789 | FACS (1:200) |
| Antibody | anti-CD11c-biotin (N418) (Armenian Hamster monoclonal) | Biolegend | Cat# 117304; RRID:AB_313773 | FACS (1:200) |
| Antibody | anti-CD11c-FITC (HL3) (Armenian Hamster monoclonal) | BD Pharmingen | Cat# 553801; RRID:AB_395060 | FACS (1:200) |
| Antibody | anti-CD19-biotin (1D3) (Rat monoclonal) | BD Pharmingen | Cat# 553784; RRID:AB_395048 | FACS (1:200) |
| Antibody | anti-CD19-FITC (MB19-1) (Mouse monoclonal) | eBioscience | Cat# 11-0191-85; RRID:AB_464966 | FACS (1:200) |
| Antibody | anti-CD25-eF450 (PC-61.5) (Rat monoclonal) | eBioscience | Cat# 48-0251-82; RRID:AB_10671550 | FACS (1:200) |
| Antibody | anti-CD25-FITC (7D4) (Rat monoclonal) | BD Pharmingen | Cat# 553072; RRID:AB_394604 | FACS (1:200) |
| Antibody | anti-CD25-PE (3C7) (Rat monoclonal) | BD Pharmingen | Cat# 553075; RRID:AB_394605 | FACS (1:200) |
| Antibody | anti-CD28-PE-CY7 (E18) (Mouse monoclonal) | Biolegend | Cat# 122014; RRID:AB_604079 | FACS (1:200) |
| Antibody | anti-CD44-PE (IM7) (Rat monoclonal) | eBiosciene | Cat# 12-0441-83; RRID:AB_465665 | FACS (1:200) |
| Antibody | anti-CD44-APC (IM7) (Rat monoclonal) | eBioscience | Cat# 17-0441-83; RRID:AB_469391 | FACS (1:200) |
| Antibody | anti-CD44-APC-eF780 (IM7) (Rat monoclonal) | eBioscience | Cat# 47-0441-82; RRID:AB_1272244 | FACS (1:200) |
| Antibody | CD45.1-PerCP-Cy5.5 (A20) (Mouse monoclonal) | eBioscience | Cat# 45-0453-82; RRID:AB_1107003 | FACS (1:200) |
| Antibody | CD45.2-APC-eF780 (104) (Mouse monoclonal) | eBioscience | Cat# 47-0454-82; RRID:AB_1272175 | FACS (1:200) |
| Antibody | anti-CXCR4-PE (2B11) | BD Pharmingen | Cat# 12-9991-82; RRID:AB_891391 | FACS (1:100) |
| Antibody | anti-DX5-biotin (Rat monoclonal) | eBioscience | Cat# 13-5971-85; RRID:AB_466826 | FACS (1:200) |
| Antibody | anti-DX5-FITC (Rat monoclonal) | Biolegend | Cat# 108906; RRID:AB_313413 | FACS (1:200) |
| Antibody | anti-GR-1-biotin (RB6-8C5) (Rat monoclonal) | Biolegend | Cat# 108404; RRID:AB_313369 | FACS (1:200) |
| Antibody | anti-LFA-1-PE (M17/4) (Rat monoclonal) | BD Bioscience | Cat# 12-0111-82; RRID:AB_465544 | FACS (1:200) |
| Antibody | anti-mouse IgG1-biotin (A85-1) (Rat monoclonal) | BD Pharmingen | 553441; RRID:AB_394861 | FACS (1:100) |
| Antibody | anti-MYC-AF647 (Y69) (Rabbit monoclonal) | Abcam | Cat# ab190560; RRID:AB_2876372 | FACS (1:400) |
| Antibody | anti-NK1.1-biotin (PK136) (Mouse monoclonal) | eBioscience | Cat# 13-5941-85; RRID:AB_466805 | FACS (1:200) |
| Antibody | anti-NK1.1-FITC (PK136) (Mouse monoclonal) | Biolegend | Cat# 108706; RRID:AB_313393 | FACS (1:200) |
| Antibody | anti-pS325-OXSR1/pS383-STK39 (sheep polyclonal) | MRC-PPU | Cat# S670B; RRID:AB_2876373 | IB (1 µg/ml) |
| Antibody | anti-PTCRA (2F5) (Mouse monoclonal) | BD Pharmingen | Cat# 552407; RRID:AB_394381 | FACS (1:100) |
| Antibody | anti-pS235/pS236-S6-AF488 (D57.2.2E) (Rabbit monoclonal) | Cell Signaling | Cat# 5317; RRID:AB_10694920 | FACS (1:50) |
| Antibody | anti-TCRβ-bio (H57-597) (Armenian Hamster monoclonal) | eBioscience | Cat# 13-5961-85; RRID:AB_466820 | FACS (1:200) |
| Antibody | anti-TCRβ-FITC (H57-597) (Armenian Hamster monoclonal) | Biolegend | Cat# 109206; RRID:AB_313429 | FACS (1:200) |
| Antibody | anti-TCRβ-APC (H57-597) (Armenian Hamster monoclonal) | Tonbo | Cat# 20–5961 U100, RRID:AB_2621612 |
FACS (1:200) |
| Antibody | anti-TCRγδ-biotin (UC7-13D5) (Armenian Hamster monoclonal) | eBioscience | Cat# 13-5811-82; RRID:AB_466684 | FACS (1:200) |
| Antibody | anti-TCRγδ-FITC (UC7-13D5) (Armenian Hamster monoclonal) | Biolegend | Cat# 107504; RRID:AB_313313 | FACS (1:200) |
| Antibody | anti-TCRγδ-PE (GL3) (Armenian Hamster monoclonal) | eBioscience | Cat# 12-5711-82; RRID:AB_465934 | FACS (1:200) |
| Antibody | anti-Thy1.2-BV605 (53–2.1) (Rat monoclonal) | Biolegend | Cat# 140317; RRID:AB_11203724 | FACS (1:500) |
| Antibody | anti-Thy1.2-FITC (53–2.1) (Rat monoclonal) | Biolegend | Cat# 140304; RRID:AB_10642812 | FACS (1:500) |
| Antibody | anti-Thy1.2-APC (53–2.1) (Rat monoclonal) | eBioscience | Cat# 17-0902-83; RRID:AB_469423 | FACS (1:500) |
| Peptide, recombinant protein | Streptavidin-PE | Biolegend | Cat #: 405204 | FACS (1:200) |
| Peptide, recombinant protein | Streptavidin-PerCP | Biolegend | Cat #: 405213 | FACS (1:200) |
| Peptide, recombinant protein | mouse CXCL12 | R and D systems | Cat # 460-SD-010/CF | (500 ng/ml) |
| Peptide, recombinant protein | mouse ICAM1-Fc | R and D systems | Cat # 796-IC-050 | Transwell assay (500 ng/ml); Adhesion Assay (25 µg/ml) |
| Commercial assay or kit | Foxp3/Transcription factor staining buffer set | eBioScience | Cat # 00-5523-00 | |
| Chemical compound, drug | Tamoxifen | Millipore-Sigma | Cat # T5648 | |
| Software, algorithm | Graphpad Prism 8 | Graphpad | RRID:SCR_002798 | |
| Software, algorithm | Flowjo 10 | Flowjo | RRID:SCR_008520 | |
| Other | 7AAD | Merck | Cat # SML1633 | |
| Other | FxCycle Violet Stain | Thermo Fisher | Cat # F10347 | (1:1000) |
| Other | LIVE/DEAD NearIR | Thermo Fisher | Cat # L10119 | (1:500) |
| Other | Zombie Aqua | Biolegend | Cat # 423102 | (1:500) |