Table 1.

Gradient conditions for chromatographic separation for DNA-TG quantification

Time segment	Time (min)	Flow rate (μL/min)	Mobile phase
			%A*	%B†
1	Initial	0.35	0	100
2	1.5	0.35	0	100
3	1.8	0.45	0	100
4	2.7	0.45	0	100
5	3.2	0.45	70	30
6	3.7	0.45	70	30
7	3.9	0.45	0	100

*0.1% formic acid in distilled water; ^†160 mM formic acid and 10 mM ammonium formate in 95% acetonitrile.

Abbreviation: DNA-TG, DNA-incorporated 6-thioguanine.