Figure 4.

Sodium Dodecyl Sulfate (SDS)-PAGE and Capsid Ratio Analysis of AAV9 Produced in Sf9 and ExpiSf9 Cells Using Capillary Electrophoresis SDS

(A) Purified AAV8 and AAV9 from both systems were assessed by SDS-PAGE gel stained with SYPRO Red. Lane 1, AAV8 produced in Sf9 cells (~2.6 × 10¹¹ vg); lane 2, AAV8 produced in ExpiSf9 cells (~2.6 × 10¹¹ vg); lane 3, AAV9 produced in Sf9 cells (~1.0 × 10¹¹ vg); lane 4, AAV9 produced in ExpiSf9 cells (~1.0 × 10¹¹ vg). (B) Chromatogram showing the overlay of the AAV9 samples produced in either Sf9 (red) or ExpiSf9 (blue) cells. Bottom table shows the number of VP1, VP2, and VP3 proteins per AAV9 particle produced in either Sf9 or ExpiSf9 cells.