Fig. 3. Analysis of ROS and lipid ROS during non-canonical pyroptosis in genetic models targeting the pyroptosis pathway.

a–b Cell death and lipid ROS accumulation in casp11^−/− BMDMs and WT 8 h after LPS transfection of LPS-primed cells. c–e Cell death, lipid ROS and ROS in gsdmd^−/− BMDMs during non-canonical pyroptosis. f Cleavage of WT and gsdmd^I105N/I105N. g–h Lipid ROS and cell death of gsdmd^I105N/I105N during non-canonical pyroptosis. i Representative fluorescent microscopy images of WT, casp11^−/−, gsdmd^−/− and gsdmd^I105N/I105N stained with C11-BODIPY and DRAQ7 5 h after LPS transfection. All bars are mean ± SEM of three biological replicates. One-way ANOVA, *p < 0.05, **p < 0.01.