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. 2020 Oct 27;11:5426. doi: 10.1038/s41467-020-19197-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Flow cytometry of primary human monocyte differentiation into macrophages with eLDL ± TNFα treatment. b Quantification of the mean fluorescence intensity (MFI) of flow cytometry results. The relative level represents normalization to negative control−/−(no GM-CSF/no treatments), (mean ± S.D., n = 3 independent wells from two monocyte isolations, CD206: ***P < 0.0001; CD80: *P = 0.0397, **P = 0.0267; CD36: *P = 0.0187, **P = 0.0143, compared to control +/− =GM-CSF control/no treatments by one-way ANOVA and Tukey post hoc test). c Characterization of primary monocytes derived macrophages in perfused TEBVs with GM-CSF pretreatment and addition of eLDL for 96 h with or without 8 h TNFα treatment; fluorescence images of, monocytes-cell tracker red, CD80 + monocytes-green (scale bar 100 μm). d Quantification of mean fluorescence intensity (MFI) per cell of CD80 + monocytes (green signal) based on the images in panel (c) (mean ± S.D., n = 3 TEBVs, *P = 0.034, **P = 0.00066, ***P < 0.0001 compared to negative control by one-way ANOVA and Tukey post hoc test).