Table 2.

Results of computational protein–protein interaction experiments predicting the impact of single residue replacements, relative to human ACE2 residues, at critical binding sites with SARS-CoV-2 receptor-binding domain.

Mutation	ΔΔG (kcal/mol)a
Y41H	1.929
Q42E	0.954
M82T	0.938
D38E	0.651
Q24L	−0.753
H34L	−0.566
H34Y	−0.139
D30E	0.692

^aMutations were analyzed with the SSIPe server (https://zhanglab.ccmb.med.umich.edu/SSIPe/) and PDB file 6M0J.