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. 2020 Oct 27;11:5414. doi: 10.1038/s41467-020-18955-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Principal component (PC) analysis of HumanMethylationEPIC profiles of parental (green triangle), iso-H3.3-WT (blue rectangles) and iso-H3.3-G34W (red circle) HeLa cells. Each data point represents an independent clone. b Methylation levels of top 2000 probes associated with each of the first three principal components in one parental (green), four iso-H3.3-WT (blue) and four iso-H3.3-G34W cells (red). c Scatter plot of average individual CpG probe methylation of four iso-H3.3-WT (x-axis) vs. four iso-H3.3-G34W (y-axis) clones. Color dots indicate incremental delta means of methylation, with orange representing >0.5 delta-mean. d Fractions of HumanMethylationEPIC probes differentially methylated between iso-H3.3-G34W and iso-H3.3-WT HeLa cells, by direction of DNA methylation change (light gray–hypomethylation, dark gray–hypermethylation). e Enrichment (positive values) or depletion (negative values) of major gene model features among differentially methylated probes. OR, odds ratio. f Genome browser snapshot displaying DNA methylation levels of CpGs in the vicinity of the TNFSF11 locus in parental (green), iso-H3.3-WT (blue) and iso-H3.3-G34W cells (red). Points represent methylation values of individual CpGs and the lines depict LOESS curves with degree 1 and span 0.5.