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. 2020 Oct 27;11:5376. doi: 10.1038/s41467-020-19175-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Heatmap representing the VAFs analysed by ddPCR from tumour DNA, and CSF and plasma ctDNA samples obtained before surgical resection for the 13 patients. Colour legend for mutant allelic frequencies (VAFs) is shown. VAFs values <10% are indicated. Not detected (ND). b Distribution of CSF ctDNA VAF. Outliers identified using ROUT method are highlighted with a grey circle and selected as High CSF ctDNA.