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. 2020 Oct 27;10:18315. doi: 10.1038/s41598-020-75026-4

Figure 6.

Figure 6

Biochemical analysis of hypothalamic synaptic and canonical Wnt signalling proteins. Western blot analysis for (a) female degus (b) male degus. Densitometric analysis hypothalamic PSD95, SYP, and NR2B proteins of (c) female degus (d) male degus. Densitometric analysis of hypothalamic total β-catenin, ratio pY216-GSK3β, and ratio pS9-GSK3β of (e) female degus (f) male degus. Data were analysed statistically using one-way ANOVA, with the p-value indicated at the top of each figure. Different letters above bars show statistical differences between the same protein across the stress treatments (Fisher’s LSD post hoc test). Results are expressed as mean ± SE (n = 3). a.u: arbitrary units. Control (CTL), Partial Isolation (PI), Chronic Isolation (CI), and Re-socialization (CI-R) treatment groups.