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. 2020 Oct 27;10:18300. doi: 10.1038/s41598-020-75171-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Time-dependent MSI1 and KLF4 expression patterns in the YFP⁺ crypts after 12 Gy TBI of the Bmi1^Ctrl mice treated according to protocol 1 (Supplementary Fig. 1A). (A) Representative IF images of DAPI, YFP, MSI1, and KLF4 staining in the PSI crypts at 0, 6, 24, 48, 72 and 96 h after irradiation obtained under a fluorescence microscope. The scale bar represents 20 µm. (B) Quantification of the percentage of YFP⁺, MSI1⁺ or KLF4⁺ cells in the YFP⁺ crypts. (C) Quantification of the percentage of YFP⁺ cells costained with MSI1, KLF4 or MSI1 and KLF4 together. Data are represented as the mean ± SD, 20 YFP⁺ crypts were quantified per mouse, and n = 3 mice per group. *p < 0.05, **p < 0.01 and ***p < 0.001 by one-way ANOVA. (D) qRT-PCR analysis of Klf4 expression in the FACS-sorted YFP⁺ cells isolated from the Bmi1^Ctrl mice treated according to protocol 1 (Supplementary Fig. 1A) at 0, 6, 24, 48, 72 and 96 h after irradiation. The sham mice were used as a control. IR, irradiated group (12 Gy TBI). Data are represented as the mean ± SD, n = 3 mice per group. *p < 0.05, **p < 0.01 and ***p < 0.001 by one-way ANOVA.