Fig. 4.

Biotin induced levels of Mfn2 containing mitochondria under ER stress are associated with ER. Effect of tunicamycin (Tm)-induced ER stress in the presence and absence of biotin supplementation on the co-localization (yellow) between mitochondrial fusion marker Mfn2 (red) and ER marker-PDI (green) were evaluated using confocal microscopy. The merged images obtained from the corresponding red and green channels are represented in the last column. The scale bar in the bottom merged image indicates a distance of 20 μm. The experiment was performed on three independent cell culture preparations (n = 3) with consistent outcomes and the outcome of one of the trials is represented