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. 2020 Jun 9;25(6):899–908. doi: 10.1007/s12192-020-01124-x

Table 1.

Primers used for quantitative real-time PCR

Gene symbol Forward primer (5′-3′) Reverse primer (5′-3′) Ref
Human ANP AAGAAAGCACACCAACGCAG GATGGTGACTTCCTCGCCTC Primerbank
Human BNP CTGATCCGGTCCATCTTCCT TGGAAACGTCCGGGTTACAG Primerbank
Human β-MHC CCAAGTTCACTCACATCCATCA AGTGGCAATAAAAGGGGTAGC Primerbank
Human HTR2A CTTTGTGCAGTCTGGATTTACCT ACTGATATGGTCCAAACAGCAAT Designed in-house
Human GAPDH GGCTGTTGTCATACTTCTCATGG GGAGCGAGATCCCTCCAAAAT Primerbank
Mouse Anp TCTTCCTCGTCTTGGCCTTT CCAGGTGGTCTAGCAGGTTC (Luo et al. 2017)
Mouse Bnp TGGGAGGTCACTCCTATCCT GGCCATTTCCTCCGACTTT (Luo et al. 2017)
Mouse β-Mhc CGGACCTTGGAAGACCAGAT GACAGCTCCCCATTCTCTGT (Luo et al. 2017)
Mouse HTR2A TAATGCAATTAGGTGACGACTCG GAGGCTTCGGAAGTGTTAGCA Designed in-house
Mouse Gapdh TGTAGACCATGTAGTTGAGGTCA AGGTCGGTGTGAACGGATTTG (Luo et al. 2017)
Rat Anp GAAGATGCCGGTAGAAGATGAG AGAGCCCTCAGTTTGCTTTTC (Luo et al. 2017)
Rat Bnp CTGGAGACTGGCTAGGACTTC GGTGCTGCCCCAGATGATT (Luo et al. 2017)
Rat β-Mhc GCCCCAAATGCAGCCAT CGCTCAGTCATGGCGGAT (Luo et al. 2017)
Rat HTR2A GCTGGGTTTCCTTGTCATGC ACAGATATGGTCCACACGGC Designed in-house
Rat Gapdh TGACAACTCCCTCAAGATTGTCA GGCATGGACTGTGGTCATGA (Luo et al. 2017)