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Quantitative measurements of Py-VPB (10 μM) fluorescence changes after addition of different concentrations of H2O2. (A) Fluorescence emission spectra of Py-VPB after addition of different concentrations of H2O2 (0–100 μM). (B) Plot of fluorescence intensity ratio changes (I480/I600) after incubation with increasing amounts of H2O2. Inset: Linear regression plot of fluorescence intensity ratio change (I480/I600) as a function of the concentration of H2O2 (0–45 μM). Ex = 380 nm.
