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(A-C) Confocal imaging of exogenous H2O2 in HeLa cells (A: 0 μM; B: 100 μM; C: 200 μM). (D) The ratio of channel 2/channel 1 fluorescence intensity when incubated with different concentrations of H2O2. The relative fluorescence intensity was analyzed by the ImageJ software. Every data point represents the mean of five fields of cells. Ex = 405 nm, channel 1: 580–630 nm (Py-VPB), channel 2: 450–500 nm (Py-VP). Scale bar = 20.0 μm.
