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. 2020 Oct 14;14:581915. doi: 10.3389/fnins.2020.581915

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Copyright © 2020 Asada, Hayashi, Murakami, Kikuiri, Kaneko, Yuan and Takagi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Representative images of 5mC immunostaining 30 min after start of treatment. The scale bar shows 50 μm. (B) Changes in the ratio of 5mC-positive neurons to total cells (Hoechst-positive cells) after start of NMDA (closed triangle) or vehicle (open circle) treatment. Images of random four areas (each area of 1.3 mm²) of each well were taken, and 302–1,250 cells were counted in each well. Results are the means ± SD (n = 7 independent experiments). *Significant difference against the control group of the same time point (p < 0.05). NT, non-treated group.