FIGURE 6.

(A,C) Effects of DNMT inhibitors 5AdC (10 μM; A) and RG108 (100 μM; C) on NMDA-induced cell injury. Representative images of PI staining are shown. The scale bar indicates 50 μm. (B,D) PI-positive cells were counted as dead cells and are shown as the ratio to total cells (Hoechst-positive cells). Counted cell number was 933–1,697 (B) and 736–1,087 (D) in each well. (E,F) Effects of DNMT inhibitor RG108 (100 μM) on NMDA-induced DNA methylation 30 min after start of treatment. (E) 5mC-positive cells were counted and are shown as the ratio to total cells (Hoechst-positive cells). Counted cell number was 731–1,049 (F) in each well. The scale bar shows 50 μm. Images of random four areas (each area of 1.3 mm²) of each well were taken. Results are the means ± SD (n = 4–5 independent experiments). PI, propidium iodide; CTL, control group.