FIGURE 3.

Modulation of GABA-induced [³H]EBOB displacement in recombinant α6β3, α6β3γ2 and α6β3δ GABA_A receptors expressed in HEK293 cells. (A) Displacement curves of [³H]EBOB (1 nM) binding as % control with 5 concentrations of humulone in the presence of GABA (3 μM). (B–D) GABA (3 μM)-induced [³H]EBOB (1 nM) binding as % control with humulone (1 μM) in the presence or absence of EtOH (30 mM). Control is the maximal [³H]EBOB binding in the presence of 3 μM GABA alone. All values represent the mean ± SEM, n = 3–6, measured in triplicates; n.s for the non-significance of difference from the corresponding control value (One-way ANOVA followed by Tukey’s post hoc test).