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(A) Incorporation of TAMRA-C6-AF into scFv in response to a UAG codon in a cell-free translation system. (B) The reaction model of TAMRA-scFv towards antigen. Trp residues, Trp33H, Trp36H, Trp47H, Trp103H, and Trp35L are colored green. (C) Antigen-dependent fluorescence enhancement of TAMRA-labeled anti-BGP scFv upon reaction with BGP-C7. The figure was reproduced with permission from Ref. [106].
