FIGURE 2.

(A) Comparative images of cryo-fixed and chemically fixed brain tissue imaged by electron microscopy, with ECS indicated in blue. It is clear that cryofixation yields better preserved ECS that is more voluminous and heterogeneous in terms of intercellular distances. (B) Comparison of α assessed in brain tissue that is cryo-fixed and tissue that is chemically fixed. Cryofixation yields comparable results to RTI measurements, whereas chemical fixation causes cellular swelling and a severe underestimation of α. From Korogod et al. (2015), with permission.