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. 2020 Oct 14;11:551256. doi: 10.3389/fmicb.2020.551256

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Copyright © 2020 Oliveira, Medina-Alarcón, Singulani, Fregonezi, Pires, Arthur, Fusco-Almeida and Mendes Giannini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative confocal microscopy images. P. brasiliensis was marked with CFSE (green) and C. albicans with Calcofluor white (blue). C. albicans (CA) and P. brasiliensis (PB) mono-species biofilms formed for 48 and 144 h, respectively. For dual-species biofilms, P. brasiliensis previously treated with CFSE was added to 12 h preformed C. albicans biofilm, and incubated for 120 h. (A) Dual-species biofilms: overlay of CA and PB (A1), CA (A2), PB (A3) and zoom in image showing dual-species interaction (A4); (B) Mono-species biofilms: CA biofilm (B1) and PB biofilm (B2).