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. 2020 Oct 27;10:18400. doi: 10.1038/s41598-020-75376-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Terminal Complement complex, C5b-9, deposition and co-localization with PTX3 in first PBx of patients subsequently developing prostate cancer (group A) or not (group B). PTX3 protein expression (green; A and E) and C5b-9 deposition (red; B and F) were evaluated in the first PBx by double label immunofluorescence and confocal microscopy as detailed in Materials and Methods. TO-PRO-3 was used to stain nuclei (blue; C and G). Merged images (D and H) do not demonstrate any co-localization of the two protein signals. Mean fluorescence intensity (MFI) quantification confirmed the absence of C5b-9 deposition in both groups (I). Bar length = 50 µ.