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. 2020 Oct 27;10:18400. doi: 10.1038/s41598-020-75376-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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C3a and C5a receptors protein expression and co-localization with PTX3 in first PBx of patients subsequently developing prostate cancer (group A) or not (group B). UPPER PANEL: Protein expression of PTX3 (green; A and E) and C3a receptor (red; B and F) were evaluated in the first PBx by double label immunofluorescence and confocal microscopy as detailed in Materials and Methods. TO-PRO-3 was used to stain nuclei (blue; C and G). Merged images (D and H) demonstrate the co-localization of the two protein signals (yellow). Mean fluorescence intensity (MFI) quantification confirmed a statistically significant higher C3a receptor expression in group A compared to group B (I). Bar length = 50 µ. BOTTOM PANEL: Protein expression of PTX3 (green; A and E) and C5aR1 (red; B and F) were evaluated in the first PBx by double label immunofluorescence and confocal microscopy. TO-PRO-3 was used to stain nuclei (blue; C and G). Merged images (D and H) demonstrate the co-localization of the two protein signals (yellow). Mean fluorescence intensity (MFI) quantification confirmed a statistically significant higher C5a receptor expression in group A compared to group B (I). Bar length = 50 µ.