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. 2020 Oct 27;10:18400. doi: 10.1038/s41598-020-75376-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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C1q and C5b-9 deposition and CD59, C3a and C5a receptor expression and their co-localization with PTX3 in second biopsy of patients who developed prostate cancer (group A) or not (group B). PTX3 (A, E, I, M, Q), C1q (B), C5b-9 (F), CD59 (J), C3a (N) and C5a receptor (R) proteins were investigated by double-label immunofluorescence and confocal microscopy as detailed in Materials and Methods. TO-PRO-3 was employed to counterstain nuclei (blue; C, G, K, O, S). Merged images (yellow; D, H, L, P, T) demonstrated the co-localization (green) of C1q (D), CD59 (L), C3a (P) and C5aR1 (T) with PTX3, whereas no co-localization was evident for C5b-9 (H). Bar length = 50 µ.