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. 2020 Oct 27;3:621. doi: 10.1038/s42003-020-01294-5

Fig. 6. Impact of HSA-heme on cell signaling.

Fig. 6

a A multi-channel reporter cell line from Jurkat T cells expressing reporter genes under control of NF-κB, NFAT, and AP-1 promoter elements were used to study the impact of HSA-heme on prominent signaling pathways. Bar graph shows the expression of the transcription factors NF-κB, NFAT, and AP1 in presence of 10% FSC, HSA, HSA-heme or transferrin analyzed by flow cytometry. Bar graph show resting cells and cells which were stimulated with a combination of CD3 and CD28 antibodies (5 µg/ml each). Graph show means ± SEM of MFI (n = 4). Only significant differences are indicated (one-way ANOVA followed by Tukey’s multiple comparison test).