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. 2020 Oct 27;3:621. doi: 10.1038/s42003-020-01294-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Phenotype of DCs generated in the presence of HSA-heme. Monocytes were differentiated into DCs in presence of 10% FCS, 1% HSA or 1% HSA-heme and analyzed after 7 days for expression of typical cell surface markers (filled histograms) compared to the isotype control (dotted histograms) via flow cytometry. Data shown are representative of four independent experiments with four different donors. b HSA-heme modulates cytokine production of DC upon stimulation with LPS (mature DCs). Supernatants were collected from DCs and mature DCs and analyzed on day 7 (n = 3). Means ± SEM are shown. *P < 0.05 (one-way ANOVA followed by Tukey’s multiple comparison test).