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. 2020 Aug 20;123(9):1445–1455. doi: 10.1038/s41416-020-01032-y

Fig. 4. E2F7 is a direct transcriptional activator of EZH2 expression.

Fig. 4

a Predicted sequences of E2F7 motif in EZH2 promoter by JASPAR. b ChIP-qPCR analysis of E2F7 levels at different regions of EZH2 promoter in LN229 cells. c ChIP-qPCR analysis of E2F7 levels at EZH2 promoter in LN229 and U251 cells expressing shCtrl or shE2F7. d Luciferase promoter/reporter constructs were created containing the truncated wild EZH2 promoter or E2F7 motif mut; then, the EZH2 promoter/reporter constructs were co-transfected with a shE2F7 or with an empty vector (shCtrl) into HEK293FT cells, and luciferase activity was evaluated 24 h later. e The mRNA levels of EZH2 in E2F7-silenced cells were determined by qRT-PCR. f The protein expression of EZH2 and H3K27me3 in E2F7-silenced cells was examined by western blot. All data are shown as the mean ± SD, **p < 0.01, ***p < 0.001.