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. 2020 Oct 27;10:18370. doi: 10.1038/s41598-020-75196-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Antimicrobial microcomposite cryogels are biodegradable and elicit minimal host inflammatory responses. H&E staining of HAGM cryogel scaffold sections explanted 4 days following subcutaneous injections in the dorsal flanks of C57BL/6 mice: (a) CP-free cryogel (0% CaO₂), (b) CP-containing (0.1% CaO₂) cryogel, and (c) CP-containing (0.1% CaO₂) cryogel contaminated with P. aeruginosa. H&E staining highlights the macroporous polymeric network of cryogels (interconnected dark blue fibers), infiltrated leukocytes (dark blue dots), fibrin formation (purple), and surrounding tissues (cryogel-free). Masson's trichrome (MT) (d) and H&E (e) staining of square-shaped implants explanted 2 months following subcutaneous injections in the dorsal flanks of C57BL/6 mice: CP-free (0% CaO₂) and CP-containing (0.2% CaO₂) cryogels (dimensions: 4 mm × 4 mm × 1 mm). The yellow doted lines indicate the boundary between the cryogel and the host tissue. Images are representative of n = 5 samples per condition.