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. 2020 Oct 27;10:18356. doi: 10.1038/s41598-020-75443-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Ex vivo imaging and imaging of sections. (a) Representative images of tumors with surrounding tissue: photos, NIR pHLIP ICG fluorescent images, overlay of photos with NIR pHLIP ICG fluorescent images, and images of HE-stained sections obtained from the same tumor-muscle pieces are shown. (b) Bright field image of HE-stained section and NIR pHLIP ICG fluorescent images of the adjacent non-processed section obtained on IR scanner shown in monochrome (green) and 16-color (white and red are the highest intensity, and black and dark blue are the lowest intensity) presentations. T indicates tumor; M indicates muscle, and S indicates stroma. (c) Magnified bright field images (using 20 × objective) of tumor (T), muscle (M1 and M2), tumor stroma (TS1, TS2 and TS3) of HE-stained section are presented in panel (b).