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. 2020 Oct 27;10:18351. doi: 10.1038/s41598-020-75186-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Stratified isolation of mouse colon for microenvironmental cellular analysis. (a) Representative images of hematoxylin and eosin (H and E) staining of whole colon, peeled layers containing mucosa and submucosa, and muscularis externa (MEx) are shown. (b) Representative images of H and E staining of de-epithelialized mucosa, muscularis mucosae (MM) with submucosa, and crypt layer are shown. Bar, 100 μm. (c) Representative images of whole colon, mucosa, MM with submucosa, and MEx from Twist2-Cre tdTomato × Col1a2 GFP mice and stained by using EpCAM are shown. (d) Crypt region of Twist2-Cre tdTomato × Col1a2 GFP mice is shown. Bar, 100 μm. Images representative of at least three independent experiments are shown.