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. 2020 Oct 27;10:18351. doi: 10.1038/s41598-020-75186-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular profiling of stratified isolated cell populations. (a) Flow cytometry of stratified isolated cells stained with CD45, EpCAM, and gp38. The number indicates percentages of gated populations. Right data indicates the percentages of each cellular component in the colon microenvironment. Data are shown as means ± SEM. (b) Flow cytometry of cells in each layer is shown. The gp38⁺ cells in the negative-gated areas for EpCAM, TER119, and CD31 are shown. The number indicates the size (percentage) of the gated population. CD90 and CD34 expression of gated gp38⁺ cells are shown. (c) gp38⁺ cells were stained with CD90 and CD34. The numbers indicate the size (percentage) of the gated populations. CD81 expressions were shown in the right panel. All data are representative of at least three independent experiments.