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. 2020 Oct 27;10:18351. doi: 10.1038/s41598-020-75186-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Wnt2b expression is retained in cryptal mesenchymal cells through the LepR. (a) ex vivo cryptal mesenchymal cells were stimulated by leptin or lactate and the expression of cryptal stem niches genes was examined. Relative changes normalized by normalized against the expression of Gapdh were shown. Each dot represents an individual subject (n = 3 or 4) (b,c) Cryptal mesenchymal cells were sorted from control and Lepr^db/db mice. (b) The expression of cryptal stem niches genes was examined by qRT-PCR analysis. Each result was normalized against the expression of Gapdh. Each dot represents an individual subject (n = 6 or 7). (c) Colon tissues of control and Lepr^db/db mice were stained by Wnt2b and EpCAM. Representative data from 3 mice are shown. Scale bar, 50 μm. (d) The expression of Acta2a was examined by qRT-PCR analysis. Each result was normalized against the expression of Gapdh. Each dot represents an individual subject (n = 6). (e) ex vivo cryptal mesenchymal cells were stimulated by leptin and the expression of Acta2 was examined (n = 6). Data are shown as means ± SEM; *P < 0.05, ***P < 0.01. n.s. indicates not significant.