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. 2020 Oct 27;10:18351. doi: 10.1038/s41598-020-75186-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Leptin administration protects intestinal inflammation. (a) Time course of this experiment. Mice underwent DSS treatment, and leptin (1 μg/g) was administered twice daily under fasting conditions to limit intrinsic leptin. (b–d) Representative images and colon length are shown (n = 4 per group). Scale bar, 100 μm. (e) Whole colon cells are isolated and the cell number of EPCAM⁺ epithelial cells and infiltrated CD11b⁺ Gr-1⁺ neutrophils in the colon were shown. *P < 0.05, ***P < 0.01. All data are representative of two independent experiments.