Figure 6.

Histologic Changes Produced by AD13 Treatment

Histology from the aortic root at the level of the aortic sinuses in LDL-R^−/− on WSD at 30 weeks of age comparing untreated and AD13-treated (2 weeks by osmotic minipump), include quantitative measurement of plaque area on Masson trichrome staining (A), plaque macrophage area by Mac-2 staining (B), plaque collagen area by Masson trichrome (C), and platelet number by CD41 staining (D) seen per aortic root section. Values are mean ± SEM. ∗p < 0.05 versus WT and LDL-R^−/− by unpaired Student's t-tests. n = 6 for each animal group. (E) Examples illustrating Masson trichrome (left), fluorescent immunohistochemistry for Mac-2 (center), and CD41 for platelets (right) are shown for comparison to Figure 4. Bars = 150 μm. Abbreviations as in Figures 1, 3, and 4.