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. 2020 Oct 27;10:18368. doi: 10.1038/s41598-020-75414-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Changes of α7nAChR expression levels in Lung Cell Populations in Obese Mice. The α7nAChR expression levels of α7nAChR⁺ alveolar macrophages (a), α7nAChR⁺ interstitial macrophages (b) were increased after stimulation of LPS. Detailed statistical results can be seen in Supplementary Table S9. RCD group, n = 8; RCD + LPS group, n = 8; HFD group, n = 8; HFD + LPS group, n = 10. α7nAChR⁺ AM MFI: by ANOVA, post-hoc LSD-t method. P < 0.001, comparing RCD + LPS group to RCD group; P < 0.001, comparing HFD + LPS group to HFD group; P < 0.01, comparing HFD + LPS group to RCD + LPS group. α7nAChR⁺ IM MFI: by ANOVA after logarithmic transformation, post-hoc LSD-t method. P < 0.001, comparing RCD + LPS group to RCD group; P < 0.001, comparing HFD + LPS group to HFD group; P < 0.001, comparing HFD + LPS group to RCD + LPS group. RCD, regular chow diet. HFD, high-fat diet. LPS, lipopolysaccharide. IM, interstitial macrophages. Mo, monocytes. AM, alveolar macrophages. Gr, Granulocytes. MFI, median fluorescence intensity. Data were presented as Mean ± SD. ***P < 0.001; **P < 0.01.