FIG 6.

Autophagy does not protect HCMV-infected cells from apoptosis. (A) Human peripheral blood monocytes were mock or HCMV infected for 24 h, followed by treatment with 5 μM Z-VAD-FMK for 1 h and then either 50 μM Spautin-1 or vehicle control for an additional 24 h. Viability was measured by flow cytometry using PI and annexin V staining. Results are representative of 3 to 4 independent experiments using different blood donors. (B and C) Pooled results from 3 to 4 independent blood donors were plotted by percentage of cells in either the “apoptotic” or “late dead” gates following treatment with Spautin-1 or SBI with or without Z-VAD-FMK.