FIG 8.

HCMV-induced autophagy prevents RIPK3-dependent monocyte cell death. (A) Human peripheral blood monocytes were mock or HCMV infected for 24 h, followed by either treatment with 5 μM GSK’872 or cotreatment with GSK’872 and 50 μM Spautin-1 or vehicle control for an additional 24 h. Viability was measured by flow cytometry using PI and annexin staining. Results are representative of 4 independent experiments using different donors. (B and C) Pooled results from 4 independent blood donors were plotted by percentage of cells in either the “apoptotic” or “late dead” gates following treatment with Spautin-1 with or without GSK’872.